Staff Research Associate I - 121219

Under the supervision and guidance of the Principal Investigator (PI), graduate students, and postdoctoral fellows, the Staff Research Associate will perform research examining the interactions between microbial pathogens (bacteria and viruses) and cells in infected zebrafish, including projects examining inflammation and the responses of the immune system and nervous system to infection.

Tasks include: imaging infected larvae through microscopy (confocal and immunofluorescence), maintaining strains of mutant and transgenic zebrafish, maintaining and generating microbial mutants (pathogenic and non-pathogenic species), manipulation of zebrafish gene function through genome editing, and genotyping zebrafish and microbes. Responsible for laboratory management including: ordering and organization of supplies, maintenance of lab and computer equipment, training of undergraduate students, routine preparation of buffers and media, zebrafish care, and laboratory safety. Will participate in the publication of scientific manuscripts based on experiments performed (when appropriate). Perform other duties as assigned.

• Theoretical and practical knowledge of aquaculture/fisheries, genetics, and/or developmental biology; acquired through a BA or BS in biology, aquaculture/fisheries, or related field, and/or equivalent combination of experience and education.

• Experience with standard recombinant DNA research techniques and standard laboratory procedures such as genome editing, DNA cloning, and immunostaining.

• Strong communication skills, both written and verbal. Ability to clearly and concisely communicate ideas, thoughts, problems, and policies, with a strong portfolio of interpersonal skills which include the ability to interact with tact, diplomacy and sensitivity.

• Demonstrated ability to organize, set priorities, meet deadlines, and efficiently carry out multiple and varied tasks during each workday.

• Ability to proactively and appropriately facilitate the maintenance of lab supplies and equipment, maintain steady levels of lab supplies and organized records.

• Experience with microscopy, preferably confocal imaging.

• Experience with zebrafish aquaculture.

• Demonstrated experience in microbiology, with some experience in bacterial genetics.

• Familiarity with Apple and PC computers and demonstrated ability to work independently with a variety of computer applications such as word processing, database, spreadsheet, and graphics software. Willingness and ability to update and increase skills and knowledge as needed.

• Ability to keep accurate records of experimental procedures and results, to discuss them, and to prepare relevant oral and written presentations.

• Demonstrated knowledge/understanding and at least some experience with Safety Data Sheets, hazard communication and Injury and Illness Prevention Program. Familiarity with a Chemical Hygiene Plan addressing safety precautions including appropriate clothing and shoes, safe handling of chemicals, use of protective items. Willingness and ability to keep safety training current and consistent with research involvement.

• Knowledge and ability to order lab supplies and equipment, with the ability to proactively and appropriately facilitate the maintenance of all lab supplies and equipment. Knowledge of UCSD and Division policies and procedures related to purchasing preferred.

• Willingness and demonstrated ability to embrace and consistently uphold the principles of community for a collaborative work environment.

• Ability to inject zebrafish embryos at the 1-cell stage with reliable and consistent results.

• Must be willing and able to safely work with, and in proximity to, BSL2 bacteria and viruses.

• Appropriate physical strength for handling aquaculture equipment.

• Appropriate dexterity for manipulating zebrafish embryos.

• Must be willing to be flexible with work hours to include occasional evening or weekend work as required for animal care and experiments.

• Background check required.

Job offer is contingent on successful engagement in the UC COVID-19 Vaccination program (fully vaccinated with documented proof or approved exception/deferral).

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